Chromogenic TAL Assay (Ikizamini cya Chromogenic endotoxin)

Chromogenic TAL Assay (Ikizamini cya Chromogenic endotoxin)

TAL reagent ni lysifile amebocyte lysate ikurwa mumaraso yubururu ya Limulus polyphemus cyangwa Tachypleus tridentatus.

Endotoxine ni amphiphilic lipopolysaccharide (LPS) iherereye mu ngirabuzimafatizo yo hanze ya bagiteri-mbi.Ibicuruzwa byababyeyi byandujwe na pyrogene harimo na LPS birashobora gutuma umuntu akura umuriro, kwinjiza igisubizo, gutwika, kunanirwa kwingingo no gupfa mubantu.Kubera iyo mpamvu, ibihugu byo ku isi byashyizeho amabwiriza, bisaba ko ibicuruzwa byose by’ibiyobyabwenge bivugako ari sterile kandi bitari pyrogenic bigomba gupimwa mbere yo kurekurwa.Gel-yambaye TAL assay yakozwe bwa mbere mugupima bacteri endotoxine (ni ukuvuga BET).Ariko, ubundi buryo bwateye imbere muburyo bwa TAL bwagaragaye.Kandi ubu buryo ntibuzamenya gusa ahubwo buzanagereranya ahari endotoxine muri sample.

Usibye tekinike ya gel-clot, tekinike ya BET ikubiyemo tekiniki ya turbidimetric na tekinike ya chromogenic.

Bioendo, yitangiye gutahura endotoxine, nuwabigize umwuga kugirango ateze imbere chromogenic TAL assay.Bioendo^TMEC Ikizamini cya Endotoxine (End-point Chromogenic Assay) itanga igipimo cyihuse cyo kugereranya endotoxine.Turatanga kandi Bioendo^TMKC Ikizamini cya Endotoxin (Kinetic Chromogenic Assay) hamwe na microplate umusomyi wa incubation ELx808IULALXH, bishobora kwemeza kwizerwa no gukora neza mubushakashatsi bwawe.