Gukoresha Tekinike ya Chromogenic kuri Bacterial Endotoxins Ikizamini

Tekinike ya Chromogenic iri muri tekinike eshatu zirimo na tekiniki ya gel-clot hamwe na tekinike ya turbidimetricike yo kumenya cyangwa kugereranya endotoxine ikomoka kuri bagiteri ya Gram-mbi ikoresheje lysate ya amoebocyte yakuwe mu maraso yubururu bwikona (Limulus polyphemus cyangwa Tachypleus tridentatus).Irashobora gushyirwa mubikorwa nkibisobanuro byanyuma-chromogenic cyangwa kinetic-chromogenic assay ishingiye kumahame yihariye yakoreshejwe.

Ihame rya reaction ni uko: lysate ya amebocyte irimo caskade ya serine protease enzymes (proenzymes) ishobora gukoreshwa na endotoxine ya bagiteri.Endotoxine ikora proenzymes kugirango itange imisemburo ikora (yitwa coagulase), iyanyuma itera igabanywa rya substrate idafite ibara, irekura ibicuruzwa byamabara yumuhondo pNA.PNA yasohotse irashobora gupimwa kuri 405nm.Kandi kwinjiza bifitanye isano neza nubushakashatsi bwa endotoxine, noneho intumbero ya endotoxine irashobora kubarwa.