Recombinant Cascade Reagents Chromogenic Assay

Bioendo rCR Ikizamini cya Endotoxin (Recombinant Cascade Reagents Chromogenic Assay) ikoresha tekinoroji ya gene recombinant kugirango igaragaze Factor C inzira serine zymogen protease ya horsehoe crab amebocyte muri selile ya eukaryotic.Reagent ya casade irimo Factor C ishobora gukoreshwa na bagiteri endotoxine, Factor B na enzyme ya proclotting.Endotoxine ihuza kandi igahindura Factor C muburyo bukora, hanyuma igakora Factor B, nayo igakora enzyme ya proclotting.Enzyme ikora proclotting enzyme noneho ikuramo chromogenic substrate, ikarekura pNA ibara ry'umuhondo.PNA yasohotse irashobora gupimwa mumafoto kuri 405 nm.Ukurikije ibyo, kwibanda kwa endotoxine yikigereranyo gishobora kubarwa.Ikizamini cya rCR endotoxin ntabwo ikoresha ibikoresho bikomoka ku nyamaswa, irinda inkware.