Kugura ubuyobozi bwa Bioendo Impera-ngingo Chromogenic LAL Ikizamini Cyibikoresho

Ubuyobozikuri Bioendo Iherezo-Ingingo Chromogenic LAL Ikizamini Cyibikoresho:

TAL reagent, ni ukuvuga lysofile ya amebocyte lysate yakuwe mu maraso yubururu bwikona ryikigina (Limulus polyphemus cyangwa Tachypleus tridentatus), burigihe ikoreshwa mugupima endotoxine ya bagiteri.

Kuri Bioendo, dukora ibikoresho kugirango dukore amaherezo ya chromogenic TAL assay / LAL assay, ikubiyemo reagent zose zikenewe kuri BET binyuze muguhindura iterambere ryamabara, kandi tukemerera umukiriya kubona ibisubizo nyabyo mugihe gito.

Amahame yo gutondekanya amaherezo ya chromogenic hamwe na TAL reagent ni uko: reaction enzymatique ibaho mugihe endotoxine ya bagiteri ikora ibintu C, hanyuma coagulase ikabaho.Noneho coagulase igabanya substrate idafite ibara kugirango irekure ibicuruzwa byamabara yumuhondo pNA.PNA yasohotse irashobora gupimwa mumafoto kuri 405nm.Kubera ko kwinjiza bifitanye isano neza nubushakashatsi bwa endotoxine, intumbero ya endotoxine irashobora kubarwa.

EC64405, ni ukuvuga BioendoEC Ikizamini cya Endotoxin Ikizamini (End-point ya Chromogenic Assay), ni ingingo ya nyuma ya Chromogenic TAL assay / LAL assay kit ishobora gukoreshwa kugirango hamenyekane umubare wa bacteri endotoxine iri murugero.EC64405 kit yakozwe na Bioendo yemerera ibizamini 64, kandi sensitivite iri hagati ya 0.1 EU / ml kugeza 1 EU / ml.Bioendo EC64405 ikubiyemo reagent zose zikenewe kumurongo wanyuma wa chromogenic.Kubera ko natwe dufite ibikoresho bya endotoxine cyangwa pyrogen idafite ibikoresho, dushobora no gupakira ibikoresho dukurikije ibyo ukeneye.

Incubating Microplate Reader irakenewe kugirango amaherezo ya chromogenic asubire.Umusomyi wa microplate umusomyi ELx808IULALXH (moderi nshya irimo gutunganywa kandi izashyirwa ahagaragara vuba) ituma ingero zitandukanye zimenyekana muri microplate 96-icyarimwe icyarimwe kandi izasesengura endotoxine ihita kandi neza.

Iherezo-rya chromogenic LAL igeragezwa hamwe nibice bibiri byibikoresho, kimwe koresha microplate umusomyi kugirango usome ibisubizo byagereranijwe na microplate idafite pyrogen, ikindi ikoresha spekitifotoometero isanzwe kugirango isome ibisubizo byuzuye ukoresheje ibirahuri bitarangwamo endotoxine.

Lyophilized Amebocyte Lysate / LAL Reagent yakozwe na Bioendo ikozwe muri amebocyte lysate ikomoka kumaraso yinkwavu.