Imirahuri yikirahure hamwe no gutunganya depyrogenation irakenewe mugupima endotoxine kugirango tumenye neza ibisubizo byikizamini.Endotoxine ni molekuliyumu igizwe nubushyuhe bwurukuta rwinyuma rwa bagiteri zimwe na zimwe za garama-mbi, kandi zirashobora gutera uburwayi bukabije ndetse n’urupfu mu bantu iyo zihari mubicuruzwa byubuvuzi cyangwa ibikoresho.
Kugirango umenye endotoxine, isuzuma rikoresha reagent zirimo Limulus Amebocyte Lysate (LAL) cyangwa bita Lyophilized amebocyte lysate, ibivuye mu maraso y’ingona y’amaraso y’ingurube ifite uburyo bwo kwambara bukoreshwa na endotoxine.Ariko, ibirahuri by'ibirahuri bitigeze bivaho birashobora kubangamira ikizamini cya LAL ukoresheje uburyo bwo kwambara no gutanga ibisubizo byiza.Kubwibyo, ibirahuri byikirahuri bikoreshwa mugupima endotoxine bigomba kuba byanduye kugirango bikureho endotoxine iyo ari yo yose ishobora kuba ihari no gukumira ibikorwa bya LAL reagent.Ibi byemeza ko ibisubizo by'ikizamini cya endotoxine ari ukuri kandi byizewe kandi ko abarwayi batagerwaho n'ingaruka mbi za endotoxine.no guharanira umutekano wibiyobyabwenge byababyeyi muri farumasi, protines, umuco wimikorere, ADN nibindi.
Gukenera ibirahuri bitarangwamo endotoxine ikoreshwa mugikorwa cyo gusuzuma endotoxin:
Endotoxin idafite ibirahurini ikintu cyingenzi cyibizamini bya endotoxine.Utu tubirahuri twagenewe kugabanya ibyago byo kwanduza endotoxine mugihe cyibizamini, byemeza ibisubizo nyabyo kandi byizewe.
Kimwe mu bintu by'ingenzi biranga ibirahuri bitarangwamo ibirahuri bya endotoxine.Iyi miyoboro ikozwe mu kirahure cyiza cya borosilike, kizwiho kurwanya bidasanzwe kwangirika kwimiti.Ibi bituma bakoreshwa neza mugupima endotoxine, kuko bashobora kwihanganira guhura nibintu byinshi byikizamini batabangamiye cyangwa ngo banduze icyitegererezo.
Ikindi kintu cyingenzi kiranga ibirahuri bya endotoxine idafite isuku ni isuku yabo.Imiyoboro isukuwe neza kandi ihindurwe mbere yo kuyikoresha kugirango ikureho ibintu byose bishobora kwanduza.Barageragejwe kandi cyane kugirango banduze endotoxine, barebe ko badafite umubare munini wibintu byangiza.
Mubyongeyeho, ibirahuri bya endotoxine idafite ibirahuri byashizweho kugirango byoroshye gukoresha.Mubisanzwe baraboneka murwego rwubunini no kugereranya kugirango bahuze urugero rwuburyo butandukanye hamwe nuburyo bwo gupima byombi ibipimo bya endotoxine yujuje ubuziranenge hamwe na testoto ya endotoxin.Bihujwe kandi nibikoresho bitandukanye byo gutegura no gupima ibikoresho, bigatuma bahitamo byinshi kandi byoroshye muri laboratoire yo gupima endotoxine.
Muri rusange, ibirahuri bitarangwamo endotoxine bigira uruhare runini muburyo bwo kwipimisha endotoxine.Ubwubatsi bwabo bufite ireme, ubwiza, nuburyo bworoshye bwo gukoresha butuma biba ikintu cyingenzi cyibizamini bya endotoxine.
Bioendo Endotoxin idafite ibirahuri bidafite ubunini10 * 75mm, 12 * 75mm, 13 * 100mm na 16 * 100mmkubikorwa byo guhinduranya hamwe nuburyo bwo kubyitwaramo.
Endotoxine idafite ibirahuri byujuje ubuziranenge bwa endotoxine iri munsi ya 0.005EU / ml.
Endotoxin idafite ibirahuri bigomba gukoreshwa muri gel clot endotoxin yipimisha kugirango wirinde ibisubizo byiza.
Endotoxine ni urukuta rwa bagiteri rushobora kwanduza ibikoresho bya laboratoire, harimo ibirahuri.
Ikizamini cya gel clot endotoxin ikoreshwa mugushakisha ahari endotoxine muri sample.Muri ubu busobanuro, hashyizweho umwenda imbere ya endotoxine.Iyi miterere yimyenda noneho igereranwa nubugenzuzi kugirango umenye intumbero ya endotoxine.
Gukoresha ibirahuri bitarangwamo endotoxine bifasha kwemeza ko kumenya endotoxine ari ukuri.Ni ukubera ko endotoxine ishobora kwizirika hejuru yikirahure cyikirahure ikabangamira ibisubizo byubushakashatsi.
Kugirango tumenye neza ko ibirahuri bikoreshwa mu isuzuma rya gel clot endotoxin bipimisha nta endotoxine, bigomba gukaraba hamwe na detergent hanyuma bigakaraba neza n'amazi adafite endotoxine.Byongeye kandi, bigomba guhindurwa hakoreshejwe autoclaving cyangwa ubushyuhe bwumye.
Mu gusoza, gukoresha ibirahuri bya endotoxine idafite ibirahuri muri gel clot endotoxin yipimisha ni ngombwa kugirango tumenye neza endotoxine.Iyi miyoboro igomba guhanagurwa neza no guhindurwa kugirango ikureho ikintu cyose gishobora kwanduza.
Igihe cyo kohereza: Jun-02-2023
