ni uruhe ruhare rwamazi adafite endotoxine mugikorwa cyo gupima endotoxine?

Amazi adafite Endotoxine afite uruhare runini muburyo bwo kwipimisha endotoxine.Endotoxine, izwi kandi nka lipopolysaccharide (LPS), ni ibintu byuburozi biboneka mu rukuta rw'utugingo ngengabuzima twa Gram-negative.Ibi bihumanya birashobora kwangiza cyane abantu n’inyamaswa iyo bidakuwe mu buvuzi nkinkingo, ibiyobyabwenge, n’ibikoresho by’ubuvuzi.

Kugirango umenye kandi ugereranye urugero rwa endotoxine neza, ikizamini cya endotoxine gishingiye ku bushakashatsi bworoshye busaba gukoresha amazi adafite endotoxine.Ubu bwoko bwamazi buvurwa kugirango bukureho ibimenyetso byose bya endotoxine, byemeze ko ibisubizo byiza byose byatanzwe nubushakashatsi biterwa gusa no kuba hariho endotoxine mu cyitegererezo cyapimwe, kandi ntabwo ari ingaruka ziterwa n’amazi.

Gukoresha amazi ya endotoxine nayo bifasha kugabanya ibisubizo byiza bitari byo, bishobora kubaho mugihe hari urugero rwa endotoxine mumazi yakoreshejwe mubushakashatsi.Ibi birashobora gushikana kubisubizo bidahwitse, birashobora gutera gutinda kurekurwa kwibicuruzwa nibibazo byubuyobozi.

Muncamake, amazi adafite endotoxine nikintu cyingenzi mubikorwa byo gupima endotoxine, byerekana neza iki kizamini gikomeye.Mugabanye ibyago byibyiza bitari byiza no kwemeza ko ibisubizo byiza bitangwa gusa mugihe habaye kwanduza endotoxine nyayo, amazi adafite endotoxine agira uruhare runini mugukora ibishoboka kugirango ibicuruzwa byubuvuzi bitekanye kandi bifite akamaro mu gukoresha abarwayi.

Amazi ya bagiteri endotoxine
Itandukaniro riri hagati ya bagiteri endotoxine yipimisha namazi meza yo gutera inshinge: pH, endotoxine ya bagiteri nimpamvu zibangamira.

Amazi ya bagiteri endotoxine
Itandukaniro riri hagati ya bagiteri endotoxine yipimisha namazi meza yo gutera inshinge: pH, endotoxine ya bagiteri nimpamvu zibangamira.

1. pH

Byiza cyane pH kubitekerezo hagatiLAL reagentna endotoxine ni 6.5-8.0.Kubwibyo, mu kizamini cya LAL, Amerika, Pharmacopoeia y’Ubuyapani hamwe na Pharmacopoeia yo mu Bushinwa yo mu 2015 bavuga ko agaciro ka pH k’ibizamini bigomba guhinduka kuri 6.0-8.0.Agaciro pH k'amazi yo gupima bagiteri endotoxine igenzurwa muri 5.0-7.0;pH agaciro k'amazi meza yo gutera inshinge agomba kugenzurwa kuri 5.0-7.0.Kubera ko imiti myinshi idafite aside irike, pH agaciro k'amazi yo gupima bagiteri endotoxine ni nziza kubizamini bya endotoxine cyangwa ibizamini bya Lyophilized amebocyte lysate.

2. Endotoxine ya bagiteri

Ingano ya endotoxine mumazi yo gupima bacteri ya endotoxine igomba kuba byibuze munsi ya 0.015EU kuri 1ml, kandi ingano ya endotoxine mumazi yo gupima bacteri endotoxine muburyo bwo kubara igomba kuba munsi ya 0.005EU kuri 1ml;Amazi ya sterile yo gutera inshinge agomba kuba munsi ya 0.25 EU ya endotoxine kuri 1ml.
Endotoxine mumazi yo gupima bagiteri endotoxine igomba kuba mike bihagije kuburyo itagomba kugira ingaruka kubisubizo.Niba amazi ya sterile yo gutera inshinge akoreshwa aho gukoresha amazi yikizamini cya Endotoxine, kubera endotoxine nyinshi iri mumazi ya sterile yo gutera inshinge, amazi ya sterile yo gutera inshinge na superposition ya endotoxine murugero rwapimwe irashobora kubyara ibyiza, bigatera igihombo cyubukungu butaziguye. ku kigo.Bitewe no gutandukanya ibiri muri endotoxine, ntibishoboka gukoresha amazi meza kugirango atere inshinge aho gukoresha amazi yo kugenzura ibizamini bya endotoxine cyangwa ibizamini bya Lyophilized amebocyte lysate.

3. Ibintu bivanga

Amazi yo gupima bagiteri endotoxine ntagomba kubangamira reagent ya LAL, kugenzura endotoxine isanzwe na LAL;nta gisabwa amazi meza yo gutera inshinge.Amazi ya sterile yo gutera inshinge bisaba umutekano no gutekana, ariko se amazi meza yo gutera inshinge azagira ingaruka kumikorere no gutuza kwa endotoxine yo kurwanya bagiteri?Amazi ya Sterile yo gutera inshinge yongerera cyangwa abuza ikizamini cya endotoxine?Abantu bake ni bo bakoze ubushakashatsi bwigihe kirekire kuriyi.Byagenzuwe binyuze mu iperereza ko amazi meza yo gutera inshinge agira ingaruka zikomeye zo kubuza ikizamini cya LAL.Niba amazi meza yo gutera inshinge akoreshwa aho gukoresha amazi yo kwipimisha LAL, hashobora kubaho ingaruka mbi, bigatuma habaho kutamenya endotoxine, ibangamira umutekano wimiti.Bitewe nuko hariho ibintu bivanga byamazi meza yo gutera inshinge, ntibishoboka gukoresha amazi meza mugutera inshinge aho gukoresha amazi yo kugenzura ikizamini cya LAL.

Niba ubunyangamugayo bwo gukaraba amazi, uburyo bwo gukaraba n’amazi yipimisha bushobora kwemezwa, birashoboka ko igenzura ryiza mu kizamini cya Limulus ridashobora gushyirwaho ahanini ntiribaho, keretse niba ibipimo byakoreshejwe bitari bisanzwe.Kugirango tumenye neza ibisubizo by'ibizamini, tugomba:
a.Kumenyera ibipimo ngenderwaho n'inganda;
b.Koresha ibicuruzwa byujuje ubuziranenge n'ibicuruzwa bisanzwe;
c.Kora ukurikije uburyo bukoreshwa.