Ikizamini cya bagiteri endotoxine (BET) ikorerwa muri laboratoire nyinshi zigezweho mugihe cyagenzuwe nkikintu cyingenzi cyo kwirinda kwivanga.
Birakwiyetekinike ya asepticni ngombwa mugihe cyo gutegura no kugabanya ibipimo no gukora ingero.Kwambaraimyitozo hanze ya laboratoire isanzwe yo kurinda umuntu (PPE) ibisabwa ntabwo bihangayikishije keretse niba ibicuruzwa biri mu kizamini bisaba ibitekerezo by’umutekano byisesengura bitewe n'uburozi cyangwa ubwandu.Gantsigomba kuba idafite TALC, nkuko TALC ishobora kuba irimo urwego rukomeye rwa endotoxine.Shyira abasomyi, ubwogero bwamazi, hamwe nubushyuhe bwumyeikoreshwa rya sample incubation igomba kuba kumuntebe ya laboratoire kure yubushyuhe, guhumeka, no guhumeka (HVAC), kunyeganyega gukomeye, hamwe na laboratoire ishobora kugira ingaruka kubisubizo.Icyitegererezo gifata ibihe nibisabwabigomba kugenwa hanyuma bikandikwa, nibiba ngombwa, kugirango tumenye neza ko ibisubizo byikizamini bishobora gutangwa mugihe cyujuje ibisabwa.
Kurugero, niba laboratoire yakiriye Amazi yo Gutera (WFI) cyangwa icyitegererezo, igomba gukonjeshwa cyangwa irashobora kuguma mubushyuhe bwicyumba, kandi kugeza ryari?Mbere yo kwipimisha, birasabwa ko icyitegererezo cyibanze (s) kivangwa bihagije mbere yo kuvanaho ibizamini bya aliquot kugirango bisuzumwe neza cyangwa nyuma yo kuyikuramo.
Bioendo Bacterial Endotoxin Ikizamini, ubushakashatsi burimouburyo bwo kwambaraibizamini bya endotoxin hamwe naigipimo cya endotoxin igeragezaUburyo bwa gel clot uburyo bwa endotoxin isuzuma ni endotoxin yujuje ubuziranenge, ubu bushakashatsi busaba ibikoreshwa ni ugutunganya depyrogenation, nka endotoxine yubusa yubusa, imiyoboro ya dilution hamwe ninama zubusa za pyrogen;Kugereranya kwa endotoxin kwinshi bifite kinetic chromogenic endotoxin, ikizamini cya kinetic turbidimetric endotoxin, ubu bushakashatsi busaba ibikoreshwa bigomba kuba byujuje urwego rwo hejuru rwa endotoxine munsi ya0.005EU / ml.Nukuvugako, niba ibyitegererezo bivura, kontineri igomba kuba icupa rya endotoxine yubusa.
Mugupima endotoxine, kwivanga birashobora guturuka kumasoko atandukanye, nkibice bya matrix matrix, reagent, cyangwa ibikoresho.
Kugira ngo wirinde kwivanga mu bushakashatsi, ingamba zikurikira zirashobora gufatwa:
1. Gutegura Icyitegererezo: Gutegura neza icyitegererezo ni ngombwa mugupima neza endotoxine.
Icyitegererezo matrix igomba kugeragezwa neza kandi igashyirwa mubikorwa kugirango ihuze na endotoxin.
By'umwihariko, kubangamira ibintu nka lipide na proteyine bigomba kuvaho cyangwa kugabanywa hakoreshejwe tekinoroji ikwiye nko kuyungurura cyangwa centrifugation.
2. Igenzura ryiza kandi ribi: Ni ngombwa gushyiramo ibintu byiza nibibi mubisubizo kugirango ukurikirane kwivanga.
Igenzura ryiza ryemeza imikorere yikizamini, mugihe igenzura ribi ryerekana kwanduza cyangwa kwivanga mubice bigize assay.
3. Kugenzura ubuziranenge: Igenzura ryiza rigomba gukorwa kuri reagent zose, ibikoresho, namazi akoreshwa mubisubizo.
Ibi byemeza ko reagents zidafite kwanduza endotoxine kandi zikora neza.
4. Ibipimo ngenderwaho: Isuzuma rigomba kuba ryemewe kugirango ibisubizo byose bigereranywa kandi byororoke.
Ibi birimo gukoresha umurongo usanzwe kugirango uhindure isuzuma no gukoresha tekinike isanzwe yo gutegura icyitegererezo, incubation, no gutahura.
5. Kwemeza: Isuzuma rigomba kwemezwa kugirango ryemeze ko ryihariye, ryoroshye, kandi ryizewe.
Ibi bikubiyemo kugerageza ingero zitandukanye, harimo izizwiho kuba zifite endotoxine, kugirango hamenyekane neza ukuri kwukuri.
Mugukurikiza izi ngamba, kwivanga birashobora kugabanuka, kandi ibizamini bya endotoxine birashobora kugerwaho.
Igihe cyo kohereza: Ukuboza-01-2022
