KCET- Ikizamini cya Chromogenic Endotoxin Ikizamini (Ikizamini cya Chromogenic endotoxin ni uburyo bukomeye kuburugero hamwe no kwivanga.)
Ikizamini cya chromogenic endotoxine (KCT cyangwa KCET) isuzuma nuburyo bukoreshwa mukumenya ko endotoxine ihari.
Endotoxine ni ibintu byuburozi biboneka mu nkuta za selile yubwoko bumwe na bumwe bwa bagiteri, harimo na bagiteri-mbi ya bagiteri nka Escherichia coli na Salmonella.Mubisobanuro bya KCET, substrate ya chromogenic yongewe kurugero, ikora hamwe na endotoxine iyariyo yose kugirango itange ibara.
Igipimo cyiterambere ryamabara gikurikiranwa mugihe ukoresheje ecran ya ecran, kandi ingano ya endotoxine murugero ibarwa hashingiwe kuri iki gipimo.
Ubushakashatsi bwa KCT nuburyo buzwi cyane bwo kumenya endotoxine mu miti yimiti, ibikoresho byubuvuzi, nibindi bicuruzwa bihura numubiri wumuntu.Ni ikizamini cyoroshye kandi cyizewe gishobora kumenya na endotoxine nkeya, bigatuma iba igikoresho cyingenzi cyo kurinda umutekano wibicuruzwa.
TAL / LAL reagent ni lyofilize ya amebocyte lysate ikurwa mumaraso yubururu ya Limulus polyphemus cyangwa Tachypleus tridentatus.
Endotoxine ni amphiphilic lipopolysaccharide (LPS) iherereye mu ngirabuzimafatizo yo hanze ya bagiteri-mbi.Ibicuruzwa byababyeyi byandujwe na pyrogene harimo na LPS birashobora gutuma umuntu akura umuriro, kwinjiza ibisubizo byumuriro, guhungabana, kunanirwa kwingingo no gupfa mubantu.
Kubera iyo mpamvu, ibihugu byo ku isi byashyizeho amabwiriza, bisaba ko ibicuruzwa byose by’ibiyobyabwenge bivuga ko ari sterile kandi bitari pyrogenic bigomba gupimwa mbere yo kurekurwa.Gel-yambaye TAL assay yakozwe bwa mbere mugupima bacteri endotoxine (ni BET).
Ariko, ubundi buryo bwateye imbere muburyo bwa TAL bwagaragaye.Kandi ubu buryo ntibuzamenya gusa ahubwo buzanagereranya ahari endotoxine muri sample.Usibye tekinike ya gel-clot, tekinike ya BET ikubiyemo tekiniki ya turbidimetric na tekinike ya chromogenic.Bioendo, yitangiye gutahura endotoxine, nuwabigize umwuga kugirango ateze imbere chromogenic TAL / LAL.
Bioendo EC Endotoxin Ikizamini (End-point Chromogenic Assay) itanga igipimo cyihuse cyo kugereranya endotoxine.
Turatanga kandi Bioendo KC Endotoxin Yipimisha Ikizamini (Kinetic Chromogenic Assay) hamwe na microplate umusomyi wa ELx808IU-SN, ishobora kwemeza kwizerwa no gukora neza mubushakashatsi bwawe.
Ni ibihe bintu biranga Uwitekakinetic chromogenic endotoxin ikizaminigupima endotoxine murugero?
Kinetic chromogenic endotoxin yipimisha nubundi buryo bukoreshwa mugupima endotoxine mubitegererezo.Ifite ibintu byinshi:
1. Ibipimo bya Kinetic: Bisa na turbidimetric assay, kinetic chromogenic assay nayo ikubiyemo gupima kinetic.Ishingiye kumyitwarire iri hagati ya endotoxine na chromogenic substrate kugirango itange ibicuruzwa byamabara.Ihinduka ryimbaraga zamabara mugihe gikurikiranwa, ryemerera kugereranya ingano ya endotoxine murugero.
2. Ibyiyumvo byinshi: Kinetic chromogenic assay irumva cyane kandi irashobora kumenya urugero rwa endotoxine mukigereranyo.Irashobora gupima neza intumbero ya endotoxine, ndetse no kurwego rwo hasi cyane, ikemeza neza no kugereranya.
3. Urwego runini rugira imbaraga: Isuzuma rifite intera nini yingirakamaro, ryemerera gupima ibipimo bya endotoxine murwego rwagutse.Ibi bivuze ko ishobora gupima ingero hamwe ninzego zitandukanye za endotoxine, zikaba zaba nkeya kandi nini cyane bitabaye ngombwa ko habaho urugero rwinshi cyangwa kwibanda.
4. Ibisubizo byihuse: Kinetic chromogenic assay itanga ibisubizo byihuse ugereranije nuburyo gakondo.Mubisanzwe ifite igihe gito cyo gusuzuma, igufasha kwihuta no gusesengura ingero.Iterambere ryibara rishobora gukurikiranwa mugihe nyacyo, kandi ibisubizo birashobora kuboneka muminota mike kugeza kumasaha abiri, bitewe nibikoresho byabigenewe hamwe nibikoresho byakoreshejwe.
5. Automation and standardisation: Isuzuma rirashobora gukorwa hifashishijwe sisitemu zikoresha, nka microplate abasomyi cyangwa
endotoxine yihariye.Ibi bituma habaho igeragezwa ryinshi kandi ryemeza ibipimo bihamye kandi bisanzwe, kugabanya amakosa yabantu no kongera imikorere.
6. Guhuza nubwoko butandukanye bwikitegererezo: Isuzuma rya kinetic chromogenic rishobora guhuzwa nubwoko butandukanye bwikitegererezo, harimo imiti, ibikoresho byubuvuzi, ibinyabuzima, hamwe n’amazi.Nuburyo butandukanye bushobora gukoreshwa mubikorwa bitandukanye no mubikorwa aho hakenewe kwipimisha endotoxine.
Muri rusange, kinetic chromogenic endotoxin yipimisha itanga uburyo bworoshye, bwihuse, kandi bwizewe bwo kumenya no kugereranya
endotoxine mu ngero.Ikoreshwa cyane mu buhanga mu bya farumasi, ibinyabuzima, n’ubuvuzi mu kugenzura ubuziranenge n’umutekano
intego zo gusuzuma.
Igihe cyoherejwe: Nyakanga-29-2019
