Ikizamini cya Endotoxin cyakozwe na Lyophilized Amebocyte Lysate (LAL Reagent)

Ikizamini cya Endotoxin cyakozwe na Lyophilized Amebocyte Lysate (LAL Reagent)

LAL Reagents: Lyophilized amebocyte lysate (LAL) ni amazi yo mu mazi ava mu ngirabuzimafatizo (amebocytes) avuye mu gikona cya Atlantike.
TAL Reagents: TAL reagent ni amazi yo mu mazi ava muri Tachypleus tridentatus.
Kugeza ubu, umusaruro nyamukuru wa LAL / TAL reagent uri muri Amerika n'Ubushinwa.

Ikizamini cya gel clot endotoxin, ubu buryo buracyakoreshwa muburyo bwisi yose kwisi, kugirango huzuzwe ibisabwa mumutekano wimiti yatewe na muntu.
NUBU Bioendo itanga kandi igatanga gel yambaye LAL reagent harimo no kumpamvu imwe yikirahure yikizamini hamwe na viali nyinshi zipimisha.
https://www.bioendo.com/gel-kwambara-endotoxin-assay/ G01, GS44, G02, G17 na G52
Iki nigisubizo cyubukungu cyo kumenya endotoxine yujuje ubuziranenge mugupima ibiyobyabwenge.Cyane cyane kumiti yatewe inshinge cyangwa imiti yababyeyi gupima endotoxine muri WFI, API cyangwa ibiyobyabwenge byarangiye.Ikizamini cya Endotoxin nikibazo kinini cyibikorwa, gikenera umuhanga kabuhariwe kugirango uhangane na gel clot assay kugirango umenye neza reuslts.

Igisubizo cyuzuye kuriG52Ikizamini cya Endotoxin:
LAL Reagent
Kugenzura Endotoxine
BET Amazi
Endotoxin idafite inama ya pipette
Endotoxine idafite ibirahuri, harimo gukora dilution hamwe nigituba.
Igikoresho cya incubation, kugirango dusabe amazi yo kwiyuhagira cyangwa ubushyuhe bwumye.Ibikoresho byose byubushakashatsi bisaba ubushyuhe bwuzuye.
Ibikoreshwa byose kugirango ukore kuri LAL yujuje ubuziranenge bwurwego rwa endotoxine "<0.005EU / ml".
Ibidukikije bigomba kuba bihuye na endotoxine.

Witondere:
Ibikoresho bya plastiki nkibikoresho bya pipette cyangwa plaque nyinshi zikoreshwa cyane mugupima endotoxine.Pharmacopoeias ihujwe (USP / CP) isaba ko ibikoreshwa byose bya pulasitike hamwe nigituba cyikirahure bigomba kuba bitarimo endotoxine ishobora kugaragara kandi bifite akamaro kamwe ariko akenshi ntibisuzumwe, bigomba kuba bifite ibipimo bitarimo ibintu bivanga.

Nigute ushobora gukora ibizamini bya endotoxine kugirango umenye endotoxine muri sample?
Mbere ya byose, ikizamini cyo kwemeza ibimenyetso bya lysate sensitivite bigomba gukorwa.Kwemeza ibyiyumvo ni kimwe kubirango.
Kubisesengura byintangarugero, kora pre-intervention endotoxin ikizamini.
Gukoresha endotoxine yuzuye ya "test test".
Gukora igipimo ntarengwa cyo kubona uburyo urwego rwa endotoxine murugero.

Iyo ukora ikizamini cyo kwemeza ibimenyetso bya lysate sensibilité, ibisubizo ntibisanzwe, 2 Lamada idafite gel?
Reba niba uburyo bwo gutegura uburyo bwa bagiteri bugenzura endotoxine ari bwo.
Kuvanga Vortex birakenewe kuri buri diluent (reba igitabo cyamabwiriza cyangwa cyitwa insert of control standard endotoxin muburyo burambuye).
Kugenzura niba lysate reagent ihuye nubugenzuzi busanzwe bwa endotoxine.

Birasabwa gukoresha ubwogero bwamazi cyangwa ubushyuhe bwumye kugirango ubushyuhe burigihe bwa lysate reagent (urugero, incubator cyangwa agasanduku kumye ntigikora).
Mugihe cya incububasi, ibidukikije byamazi mubwogero bwamazi buhagaze kandi butajegajega, hirindwa kunyeganyega.
Witondere cyane ubwogero bwamazi kugirango uzimye pompe yamazi.

Urebye ibisubizo, fata witonze umuyoboro wikizamini cyangwa ampule yikirahure muri thermostat, uhindure buhoro dogere 180,
imiterere ya gel muri tube ntabwo izahinduka, kandi ntizanyerera, ibisubizo byanditswe nikimenyetso "+";
nta mikorere ya gel ibaho cyangwa imyenda ya gel ntishobora kuguma neza nubwo yashizweho.
Kunyerera ku rukuta ni bibi, ibisubizo byanditswe ku kimenyetso “-”.
Ikizamini cyihuta cya gel endotoxin Ikizamini ni uburyo bwo gupima gel gel endotoxin.
Mugihe cyihuta cya gel clot endotoxin, sample nziza igenzura ntabwo itanga gel?
Ubwa mbere, koresha amazi ya bagiteri endotoxine kugirango ugenzure reagate ya lysate, urebe ko abayikora nibidukikije byujuje amabwiriza;

Niba ibikoresho byujuje ibisabwa, noneho igenzura ryiza ryikitegererezo ntirishobora kubaho kubera ingaruka zo kubuza icyitegererezo, kandi icyitegererezo kigomba gutunganywa no kuvurwa.
Uburyo bukunze gukoreshwa muburyo bwo gutunganya ni dilution.