LAL Reagent cyangwa TAL Reagent kubizamini bya endotoxine

Limulus amebocyte lysate (LAL) cyangwa Tachypleus tridentatus lysate (TAL) ni amazi yo mu mazi ava mu ngirabuzimafatizo.

Kandi endotoxine ni molekile ya hydrophobique igizwe na lipopolysaccharide igizwe na membrane yo hanze ya bagiteri ya Gram-mbi.Ibicuruzwa byababyeyi byandujwe na pyrogene birashobora gutera ingaruka zikomeye nka feri, guhungabana, kunanirwa kwingingo, cyangwa urupfu.

LAL / TAL reagent irashobora kwitwara na bagiteri endotoxine na lipopolysaccharide (LPS).LAL ya endotoxine yo guhuza no kwambara niyo ituma iba ingirakamaro cyane mubikorwa byacu bya farumasi.Niyo mpamvu rero LAL / TAL reagent ishobora gukoreshwa kugirango tumenye cyangwa tugereranye endotoxine ya bagiteri.

Mbere yo kubona ko LAL / TAL ishobora gukoreshwa mugupima bagiteri ya endotoxine, inkwavu zikoreshwa mugushakisha no kugereranya endotoxine mubicuruzwa bya farumasi.Ugereranije na RPT, BET hamwe na LAL / TAL reagent irihuta kandi ikora neza, kandi nuburyo buzwi bwo gukora igenzura rikomeye ryikwirakwizwa rya endotoxine mu nganda zimiti, nibindi.

Ikizamini cya gel clot endotoxin, kizwi kandi ku kizamini cya Limulus Amebocyte Lysate (LAL), cyangwa cyitwa Lyophilized Amebocyte Lysate (LAL) ni uburyo bukoreshwa cyane mu gutahura no kubara endotoxine mu bicuruzwa bitandukanye, cyane cyane mu nganda zikoresha imiti n’ubuvuzi.Bifatwa nkigisubizo gikenewe mubijyanye no kumenya endotoxine kubera imikorere yayo no kwemerwa n'amategeko.

Ikizamini cya LAL gishingiye ku ihame ry'uko uturemangingo tw'amaraso tw’ibisimba by'amafarashi (Limulus polyphemus cyangwa Tachypleus tridentatus) birimo ibintu byambara bifata na endotoxine ya bagiteri, bikaviramo kwibumbira mu mitsi.Iyi reaction irakomeye cyane kandi yihariye kuri endotoxine, aribintu byubumara bigize membrane yo hanze ya bagiteri-mbi.

Hariho impamvu nyinshi zituma gel clot endotoxin yipimisha ifatwa nkigisubizo gikenewe mugutahura endotoxine:

1. Kwemererwa kugenzura: Ikizamini cya LAL cyemewe kandi cyemewe ninzego zibishinzwe nka Pharmacopeia yo muri Amerika (USP) na Pharmacopoeia yu Burayi (EP) nkuburyo busanzwe bwo gupima endotoxine.Kubahiriza aya mabwiriza ni itegeko mu kurinda umutekano n’ubuziranenge bw’ibicuruzwa bya farumasi.

2. Ibyiyumvo byihariye kandi byihariye: Ikizamini cya LAL gifite sensibilité yo hejuru, ituma hamenyekana urwego ruto cyane rwa endotoxine.Irashoboye kumenya intungamubiri za endotoxine munsi ya 0.01 ya endotoxine kuri mililitiro (EU / mL).Umwihariko w'ikizamini uremeza ko mbere na mbere itahura endotoxine kandi ikagabanya ibisubizo byiza-byiza.

3. Ikiguzi-Ingaruka: Ikizamini cya gel clot endotoxin isanzwe ifatwa nkigisubizo cyubukungu ugereranije nubundi buryo nka chromogenic cyangwa turbidimetric assays.Irasaba reagent nkeya nibikoresho, kugabanya ibiciro byo kwipimisha muri rusange.Byongeye kandi, kuboneka kwa LAL reagent isanzwe ku isoko bituma byorohereza laboratoire gukora ikizamini.

4. Inganda zinganda: Ikizamini cya LAL cyakoreshejwe cyane munganda zimiti nubuvuzi nkuburyo busanzwe bwo kumenya endotoxine.Nibice bigize gahunda yo kugenzura ubuziranenge mugihe cyo gukora imiti yimiti nibikoresho byubuvuzi, byemeza kubahiriza ibisabwa n'amategeko.

Ariko, birakwiye ko tumenya ko gel clot endotoxin yipimisha ishobora kuba ifite aho igarukira, nko kwivanga mubintu bimwe na bimwe nibishobora kubeshya-byiza cyangwa ibinyoma-bibi.Mubihe byihariye, ubundi buryo nka chromogenic cyangwa turbidimetric assays burashobora gukoreshwa mukuzuza cyangwa kwemeza ibisubizo byabonetse mubizamini bya LAL.