Amazi meza afite uruhare runini mugupima endotoxine

Amazi Yubusa ya Endotoxine: Gukina Uruhare Rwingenzi Mubizamini bya Endotoxine

Iriburiro:

Kwipimisha Endotoxine nikintu gikomeye mu nganda zitandukanye, harimo imiti, ibikoresho byubuvuzi, hamwe n’ibinyabuzima.Kumenya neza kandi kwizewe kwa endotoxine ningirakamaro kugirango umutekano wibicuruzwa no kubahiriza ibipimo ngenderwaho.Kimwe mu bintu by'ibanze bisabwa mu gupima endotoxine ni ugukoresha amazi adafite endotoxine.Muri iki kiganiro, tuzasuzuma akamaro k’amazi adafite endotoxine, uruhare rwayo mu gukora ibizamini bya endofoxine ya Lyophilized Amebocyte Lysate (LAL), n’akamaro ko gukoresha amazi adafite endotoxine mu kizamini cya Bacterial Endotoxin (BET).

Gusobanukirwa Endotoxine:

Endotoxine ni lipopolysaccharide (LPS) iboneka kuri membrane yo hanze ya bagiteri-mbi.Ni abahuza bakomeye b'umuriro kandi birashobora gutera ingaruka mbi iyo biboneka mubicuruzwa bya farumasi cyangwa ibikoresho byubuvuzi.Bitewe nubushobozi bwabo bwo gutera pyrogenic reaction, gutahura neza no kugereranya endotoxine ni ngombwa.

Ikizamini cya LAL Endotoxin:

Uburyo buzwi cyane mugupima endotoxine ni LAL assay, ikoresha amaraso yikigina cyamafarasiLimulus polyphemus na Tachypleus tridentatus.Lyofilized Amebocyte Lysate (LAL) reagent ikurwa mu ngirangingo z'amaraso y'ibi bikona, irimo poroteyine yuzuye ikora imbere ya endotoxine.

Uruhare rwaAmazi ya Endotoxinemu Kizamini cya LAL:

Amazi nikintu cyibanze mugutegura reagent nintambwe yo kugerageza LAL.Nubwo, urugero rwinshi rwa endotoxine iboneka mumazi asanzwe arashobora kubangamira ukuri no kwiyumvisha neza.Kugira ngo ukemure iki kibazo, amazi adafite endotoxine agomba gukoreshwa mugihe cyo kwipimisha.

Amazi adafite Endotoxine afite uruhare runini mugukora ibishoboka byose kugirango reagent zikoreshwa mu isuzuma rya LAL zitanduzwa na endotoxine.Byongeye kandi, irinda ibisubizo byiza cyangwa ibinyoma ibisubizo bibi, bityo bigatanga urugero rwa endotoxine yizewe kandi yuzuye.

Guhitamo Amazi meza yo Kwipimisha LAL:

Kugirango ubone amazi adafite endotoxine, uburyo bwinshi bwo kweza burashobora gukoreshwa.Deionisation, distillation, na osmose ihindagurika ikoreshwa muburyo bwo kugabanya endotoxine mumazi.Ubu buhanga bukuraho umwanda utandukanye, harimo na endotoxine ikomoka kuri bagiteri.

Byongeye kandi, ni ngombwa kwemeza ko ibikoresho bikoreshwa mu kubika, gukusanya, no gukwirakwiza amazi adafite endotoxine byemewe neza kandi bitarimo kwanduza endotoxine.Ibi bikubiyemo gukoresha endotoxine idafite imiyoboro, amacupa, na filteri mugihe cyibikorwa.

Akamaro k'amazi meza:

MuriIkizamini cya bagiteri Endotoxine (BET), amazi ya endotoxine, azwi kandi ku izina rya BET amazi, akoreshwa nkigenzura ribi kugirango yemeze ibyiyumvo byihariye nibisobanuro bya LAL.Amazi meza agomba kuba arimo urwego rutamenyekana rwa endotoxine, akemeza ko ibikorwa byose bipima endotoxine ikomoka gusa kuburugero rwapimwe.

Gukoresha amazi meza muri test ya endotoxine ikora nkigenzura rikomeye kugirango hemezwe imikorere ya LAL reagent, sisitemu yikizamini, nibikoresho.Iyi ntambwe yo kwemeza ningirakamaro kugirango dusuzume neza ko endotoxine ihari hamwe nubunini bwa sample yapimwe.

Umwanzuro:

Amazi adafite Endotoxine agira uruhare runini mugutahura neza kandi kwizewe kwa endotoxine mu nganda zitandukanye.Mu gupima LAL endotoxine, iremeza ko reagent zikoreshwa zitanduye, zitanga umubare wuzuye.Muri BET, amazi adafite endotoxine akora nk'igenzura, yemeza ibyiyumvo bya LAL.Mugukurikiza uburyo bukomeye bwo kweza no gukoresha ibikoresho byemewe, ubushobozi bwibisubizo byibinyoma namakosa birashobora kugabanuka cyane.

Mugihe akamaro ko gupima endotoxine gakomeje kwiyongera, uruhare rwamazi adafite endotoxine arushaho kuba ingenzi.Gukoresha uburyo bwizewe bwo kweza amazi no gushyiramo uburyo bwiza mugikorwa cyo kwipimisha bizarinda umutekano no kubahiriza ibikoreshwa mu bya farumasi, ibikoresho byubuvuzi, nibindi bikoresho byangiza endotoxine.