Recombinant Factor C (rFC) assayni uburyo bukoreshwa cyane mugutahura bagiteri endotoxine, izwi kandi nka lipopolysaccharide (LPS), Endotoxine ni igice cyibice byo hanze bya bagiteri ya Gram-mbi ishobora gutera igisubizo gikomeye mubikoko, harimo nabantu.Isuzuma rya rFC rishingiye ku gukoresha uburyo bwa genetike bwakozwe na Factor C, enzyme isanzwe iboneka mu maraso y’inkwavu kandi ifite uruhare mu nzira yo kwambara.Mubisobanuro bya rFC, reombinant Factor C ikoreshwa mugutahura ko endotoxine ihari mugupima Mugupima ibirimo insimburangingo zometse imbere ya endotoxine.Ugereranije nuburyo gakondo bwo gutahura endotoxine, nka Limulus Amebocyte Lysate (LAL) isuzuma rikoresha amaraso yinkwavu yinkwavu, isuzuma rya rFC rifatwa nkibisanzwe kandi byororoka, kuko ridashingiye ku gukoresha imiti ikomoka ku nyamaswa.Isuzuma rya rFC naryo ryangiza ibidukikije kandi rirambye, kuko rigabanya gukenera gukusanya no gukoresha inkwavu zifarashi mu gutahura endotoxine.
Isuzuma rya rFC ryemejwe n’inzego zishinzwe kugenzura, nka Pharmacopeia yo muri Amerika (USP), Pharmacopoeia y’Uburayi (EP) na Pharmacopoeia y’Abashinwa (CP) kugira ngo ikoreshwe mu gupima ubuziranenge bw’imiti n’ibikoresho by’ubuvuzi.
Ibyiza bya recombinant factor c assay
Ubushakashatsi bwa Recombinant C (rFC) butanga inyungu nyinshi kurenza uburyo gakondo bwo kumenya endotoxine, nka Limulus Amebocyte Lysate (LAL).Bimwe mubyiza byo gusuzuma rFC harimo:
1. Ibipimo ngenderwaho: Isuzuma rya rFC ni tekinoroji ya ADN ya recombinant ikoresha proteine imwe, isobanuwe nka reagent ya detection.Ibi bituma isuzuma rirushaho kuba ryiza kandi ntirishobora guhinduka ugereranije na LAL assay, ishingiye ku gukoresha uruvange rugoye rwa poroteyine zivanwa mu maraso y’inkwavu.
2. Imyororokere: Isuzuma rya rFC rifite urwego rwo hejuru rwo kororoka, kuko rikoresha poroteyine imwe, isobanuwe nka reagent.Ibi bituma habaho ibisubizo bihamye, ndetse no mubice bitandukanye hamwe na reagent nyinshi.
3. Kugabanya imikoreshereze y’inyamaswa: Isuzuma rya rFC nuburyo bwangiza ibidukikije kandi burambye bwo kumenya endotoxine, kuko bidasaba gukoresha inyamaswa nzima cyangwa ibitambo, nkibikona.
4. Ikiguzi-cyiza: Isuzuma rya rFC muri rusange rirahenze cyane kuruta LAL, kubera kugabanuka gukenewe kwinyamaswa nzima hamwe nuburyo busanzwe bwibisubizo.
5. Guhagarara: Isuzuma rya rFC rirakomeye kandi rirashobora gukoreshwa muburyo butandukanye, harimo gupima ubuziranenge bwimiti yimiti, ibikoresho byubuvuzi, nibindi bicuruzwa bishobora kuba birimo endotoxine.
6. Kwemeza amabwiriza: Isuzuma rya rFC ryemejwe ninzego zibishinzwe nka Pharmacopeia yo muri Amerika (USP), Pharmacopoeia yu Burayi (EP) na Pharmacopoeia y’Abashinwa (CP) kugira ngo ikoreshwe mu gupima ubuziranenge bw’imiti n’ibikoresho by’ubuvuzi.Ibi bitanga urwego rwo hejuru rwicyizere muburyo bwo kwizerwa no kwizerwa.
Kugira ngo ibyifuzo bitandukanye bishoboke, Bioendo nayo itanga kandi ikanatanga uburyo bwa gakondo bwa gel clot endotoxin test assay kit, gel gel yihuta yihuta, ibikoresho bya endotoxin bipimisha ibikoresho birimo "kinetic turbidimetric endotoxin igeragezwa ryibikoreshonakinetic chromogenic endotoxin yipimisha ibikoresho”.
Igihe cyo kohereza: Gashyantare-19-2023
