Endotoxin

Endotoxine ni ntoya ya bagiteri ikomoka kuri hydrophobique lipopolysaccharides (LPS) molekile iba muri selile yo hanze ya bagiteri-mbi.Endotoxine igizwe nuruhererekane rwibanze rwa polysaccharide, O yihariye iminyururu ya polysaccharide (O-antigen) hamwe na lipide compentent, Lipid A, ishinzwe ingaruka zuburozi.Indwara ya bagiteri yamennye endotoxine nyinshi iyo apfuye kandi iyo ikura kandi igabana.Coli imwe ya Escherichia irimo molekile zigera kuri miliyoni 2 kuri selile.

Endotoxine irashobora kwanduza byoroshye labwares, kandi kuba ihari birashobora gutanga cyane haba muri vitro ndetse no mubushakashatsi bwa vivo.Naho kubicuruzwa byababyeyi, ibicuruzwa byababyeyi byandujwe na endotoxine harimo na LPS birashobora gutera indwara yumuriro, kwinjiza ibisubizo byumuriro, guhungabana, kunanirwa kwingingo no gupfa mubantu.Kubicuruzwa bya dialyse, LPS irashobora kwimurwa binyuze muri membrane ifite ubunini bunini bwa pore mugihe cyo kuyungurura inyuma kuva mumazi ya dialyse kugeza mumaraso, ibibazo byumuriro birashobora guterwa bikurikije.

Endotoxine igaragazwa na Lyophilized Amebocyte Lysate (TAL).Bioendo yitangiye gukora ubushakashatsi, guteza imbere no gukora TAL reagent mumyaka irenga mirongo ine.Ibicuruzwa byacu bikubiyemo tekiniki zose zikoreshwa mugushakisha endotoxine, aribwo tekinike ya gel-clot, tekinike ya turbidimetric, hamwe na chromogenic.