Mu mikorere ya testi ya bacteri endotoxin, koresha amazi ya endotoxine niyo mahitamo meza yo kwirinda kwanduza

Mu mikorere yabagiteri endotoxine yipimisha, gukoresha amazi ya endotoxine ni ngombwa kugirango twanduze.Kubaho kwa endotoxine mumazi birashobora kuganisha kubisubizo bidahwitse hamwe nibisubizo byangiritse.Aha niho amazi ya Lyophilized Amebocyte Lysate (LAL) yongera amazi hamwe na testi ya bacteri endotoxine (BET).Aya mazi yabugenewe ni ngombwa mu kwemeza kwizerwa no kumenya neza ibizamini bya endotoxine mu nganda zitandukanye, harimo imiti, ibikoresho by'ubuvuzi, laboratoire z'ubushakashatsi n'ibindi.

UwitekaLAL amazi mezani amazi asukuye cyane yateguwe kugirango akoreshwe mu kizamini cya LAL kuri endotoxine.Aya mazi akora inzira ikomeye yo gukora kugirango yizere ko idafite endotoxine, ishobora kubangamira ibisubizo byikizamini.Kubura endotoxine mumazi ya LAL reagent ningirakamaro mukwemeza ibyiyumvo byihariye kandi byihariye byikizamini cya LAL, bigatuma ihitamo neza kugirango tumenye endotoxine.

Mu buryo nk'ubwo, amazi ya BET nayo ni ikintu gikomeye mu gupima bacteri endotoxine.Aya mazi yateguwe kandi arageragezwa kugirango harebwe niba adafite endotoxine nandi yanduza bishobora kugira ingaruka ku kizamini.Gukoresha amazi meza muri testoto ya endotoxine ningirakamaro kugirango ubone ibisubizo byizewe kandi byororoka, kuko bikuraho ibyago byibyiza bibi cyangwa ibibi bishobora kubaho bitewe nuko endotoxine iba mumazi asanzwe.

Akamaro ko gukoresha amazi adafite endotoxine mugupima endotoxin ntishobora kuvugwa.Ukuri nukuri kwizerwa ryibisubizo biterwa nubwiza bwamazi yakoreshejwe.Kubaho kwa endotoxine mu mazi birashobora gutuma umuntu asoma ibinyoma, bishobora kugira ingaruka zikomeye mu nganda aho kwipimisha endotoxine ari ngombwa mu kurinda umutekano n’ibicuruzwa.Kubwibyo, gushora imari muri LAL reagent cyangwa amazi ya BET ni ngombwa mugukomeza ubusugire bwibizamini bya endotoxine no kwemeza ubwiza n’umutekano wibicuruzwa.

Mu gusoza, gukoresha amazi adafite endotoxine, nk'amazi ya LAL n'amazi meza, ni ngombwa mu mikorere ya bagiteri ya endotoxine.Aya mazi yateguwe byumwihariko agamije gukuraho ibyago byo kwanduza no kwemeza neza kwipimisha endotoxine.Ukoresheje ayo mazi, inganda zirashobora kwizerwa kwipimisha endotoxine nta gutinya ibisubizo bidakwiye kubera ko mumazi ahari endotoxine.Ubwanyuma, gukoresha amazi ya LAL reagent namazi meza ni ngombwa mugukurikiza amahame yo hejuru yubuziranenge n’umutekano mu nganda aho gupima endotoxine bifite akamaro kanini.

Mugihe ukora igeragezwa rya bagiteri ya endotoxine, ni ngombwa gukoresha amazi adafite endotoxine kugirango ubone ibisubizo nyabyo kandi byizewe.
Endotoxine ni ubushyuhe butajegajega bwurukuta rwa selile ya bagiteri-mbi, kandi birashobora gutera umuriro, guhungabana, ndetse no gupfa mubantu ninyamaswa.
Niyo mpamvu, ni ngombwa gukoresha amazi adafite endotoxine mugihe ukora assay.

Hariho ubwoko butandukanye bwamazi ashobora gukoreshwa mugupima kwa bagiteri endotoxine, harimo amazi ya LAL reagent, amazi ya TAL reagent, namazi hamwe no kuvura depyrogene.Buri bwoko bwamazi yashizweho kugirango tumenye neza ko endotoxine idahari, bityo bikareba niba ibisubizo byakozwe neza.

LAL reagent amazi ni amazi yapimwe byumwihariko kandi yemejwe ko adafite endotoxine.Aya mazi akunze gukoreshwa muri Lyophilized Amebocyte Lysate (LAL), nuburyo bukunze kugaragara mugutahura endotoxine.Ukoresheje amazi ya LAL reagent mubushakashatsi, abashakashatsi barashobora kwizera ko amazi ubwayo atagira uruhare mubisubizo bibi cyangwa bibi.

Mu buryo nk'ubwo, amazi ya TAL reagent ni amazi yageragejwe kandi yemejwe ko adafite endotoxine.Aya mazi akunze gukoreshwa muri Tachypleus Amebocyte Lysate (TAL), ubundi buryo busanzwe bwo kumenya endotoxine.Ukoresheje amazi ya TAL reagent mubisubizo, abashakashatsi barashobora kwizera ko amazi ubwayo atagira uruhare mubisubizo bibi cyangwa bibi.

Amazi hamwe no kuvura depyrogene nubundi buryo bwo kwemeza ko amazi akoreshwa mugupima kwa bagiteri endotoxine adafite endotoxine.Kuvura Depyrogenation bikubiyemo kuvana cyangwa kudakora pyrogène, harimo na endotoxine, mumazi.Ibi birashobora kugerwaho binyuze mubikorwa nko kuyungurura, kuyungurura, cyangwa kuvura imiti.Ukoresheje amazi hamwe nubuvuzi bwa depyrogenation mubushakashatsi, abashakashatsi barashobora kwizera ko amazi ubwayo atagira uruhare mubisubizo bibi cyangwa bibi.

None, ni ukubera iki ari ngombwa cyane gukoresha amazi adafite endotoxine muri test ya bagiteri ya endotoxine?Kubaho kwa endotoxine mumazi akoreshwa mubisubizo birashobora gutuma habaho ibisubizo bidahwitse, bishobora kugira ingaruka zikomeye kubushakashatsi no mubuvuzi.Kurugero, niba endotoxine ihari mumazi, irashobora gushikana kubisubizo byiza, byerekana ko hariho endotoxine mugihe idahari.Ibi birashobora gutuma umuntu ahangayikishwa bidakenewe no gukoresha nabi umutungo kugirango akemure ikibazo kitabaho.

Ibinyuranye, niba endotoxine iboneka mumazi ikagenda itamenyekanye, irashobora gutuma habaho ingaruka mbi zitari zo, byerekana ko endotoxine idahari mugihe koko.Ibi birashobora gutuma irekurwa ryibicuruzwa byanduye, bigashyira ubuzima bwabantu hamwe ninyamaswa.

Usibye ingaruka zishobora guterwa nukuri kubisubizo byikizamini, gukoresha amazi adafite endotoxine irashobora no kugira ingaruka kumikorere yikizamini ubwacyo.Endotoxine irashobora kubangamira reagent nibikoresho bikoreshwa mugusuzuma, biganisha kubisubizo bitizewe cyangwa bidahuye.Ukoresheje amazi adafite endotoxine, abashakashatsi barashobora kugabanya izo ngaruka kandi bakemeza ko isuzuma ryakozwe mubihe byizewe.

Ubwanyuma, kwemeza ko amazi akoreshwa mugupima kwa bagiteri endotoxine adafite endotoxine ningirakamaro mugukomeza ubusugire bwibisubizo.Haba gukoresha amazi ya LAL, amazi ya TAL reagent, cyangwa amazi hamwe no kuvura depyrogene, abashakashatsi barashobora gufata ingamba zifatika kugirango amazi atagira uruhare mubitagenda neza cyangwa bidahuye mubisubizo byubushakashatsi.Mugukora ibyo, barashobora kwigirira icyizere mubyo babonye kandi barashobora gufata ibyemezo byuzuye bashingiye kubisubizo byakozwe.