Ibiryo bidafite pyrogene birashobora gukoreshwa nta endotoxine idasanzwe, harimo inama ya pirate idafite pyrogen (agasanduku k'iposita), imiyoboro yipimisha idafite pyrogen cyangwa yitwa ibirahuri byubusa bya endotoxin, ibirahuri bidafite ibirahuri bya pirogene, microplate ya endotoxine idafite 96, na endotoxine- amazi yubusa (gukoresha amazi ya depyrogenated mugupima bagiteri ya endotoxine), endotoxine yubusa nibindi nibindi Muri byo, amazi ya bagiteri endotoxintest hakoreshejwe uburyo bwa gel clot hamwe nubushakashatsi bwa endotoxin bwuzuye mubisobanuro byose bijyanye na Pharmacopoeia (USP, EP, BP, JP n'Ubushinwa Pharmaceutica).Yerekeza kumazi meza yo guterwa hamwe na endotoxine iri munsi ya 0.015EU / ml.Noneho verisiyo yanyuma ya Pharmacopoeia, amazi meza ni munsi ya 0.005EU / ml.Ndetse ibipimo bihanitse biri munsi ya 0.001EU / ml irashobora kubyazwa umusaruro no gutangwa na Bioendo.
Amagambo y'ingenzi ajyanye na endotoxine, hamwe na endotoxine ikoreshwa kubuntu, pyrogene nubushyuhe ni ibintu bibiri bitandukanye rwose:Pyrogen: Nanone bita pyrogen cyangwa ibintu bituruka hanze.ibintu bishobora gutera kwiyongera k'ubushyuhe bw'umubiri.Ubushyuhe: Ikintu gisohora ubushyuhe.Nka gutwika imipira, amakara, nibindi.Ibyo bita "ibiribwa bitari pyrogenic" na "igisubizo cya pyrogenic" bya bamwe mubakora ibicuruzwa n'abacuruzi mubyukuri ni amazina adasanzwe kandi ayobya.Ibikwiye bigomba kuba "Pyrogen Free" na "Pyrogen Igisubizo".
Ni ukubera iki Pyrogen ikoreshwa kubusa irakenewe mugupima endotoxine, byombi gel clot endotoxin ikizamini hamwe na endotoxin yipimishije?
Nibyo, pyrogen idafite ibiribwa ningirakamaro mugukora test ya endotoxine neza kandi yizewe.Kubaho kwa pyrogene, aribintu bitera umuriro akenshi biva muri bagiteri endotoxine, birashobora kubangamira ibisubizo byikizamini kandi biganisha ku gusoma ibinyoma.Ikizamini cya endotoxine, kizwi cyane ku izina rya Limulus amebocyte lysate (LAL) cyangwa cyitwa Lyophilized amebocyte lysate (LAL), gikoreshwa mu gutahura no kugereranya ahari endotoxine ya bagiteri mu miti y’imiti, ibikoresho by’ubuvuzi, n’ibindi bicuruzwa.Ikizamini cya LAL gishingiye ku myitwarire iri hagati ya LAL reagent na endotoxine kugirango itange igisubizo cyambaye cyangwa chromogenic.Kugirango ubone ibisubizo nyabyo, ni ngombwa gukoresha ibikoreshwa bitarimo pyrogene.Pyrogene irashobora kwanduza ibikoresho bitandukanye bya laboratoire, harimoibikoresho by'ibirahure, inama za pipeti, tebes, hamwe nicyitegererezo.Niba ibirungo byanduye byitwa pyrogen bihuye na LAL reagent cyangwa ibizamini byikigereranyo, birashobora gukurura ibinyoma-byiza, biganisha ku myanzuro itari yo kubyerekeye endotoxine ihari.Ibikoresho bidafite pyrogene bikozwe kandi bipimwa byumwihariko kugirango bigabanye cyangwa bikureho pyrogene.Bahura nuburyo bukomeye bwo kugenzura ubuziranenge kugirango barebe ko bujuje ibipimo bisabwa kugirango bapimwe endotoxine.Gukoresha ibyo bikoresho byihariye bifasha kugumana ubunyangamugayo nukuri kwipimisha rya endotoxine, gutanga ibisubizo byizewe no guteza imbere umutekano wumurwayi mubikorwa bya farumasi nubuvuzi.
Igihe cyo kohereza: Ugushyingo-07-2022
