Ikizamini cya Endotoxine ni iki?

Ikizamini cya Endotoxine ni iki?

Endotoxine ni molekile ya hydrophobique igizwe na lipopolysaccharide igizwe na membrane yo hanze ya bagiteri-mbi.Zirekurwa iyo bagiteri zipfuye kandi ibibari byo hanze bigasenyuka.Endotoxine ifatwa nkabagize uruhare runini mugusubiza pyrogenic.Ibicuruzwa byababyeyi byandujwe na pyrogène birashobora gutuma umuntu akura umuriro, kwinjiza igisubizo, guhungabana, kunanirwa kwingingo no gupfa mubantu.

Ikizamini cya Endotoxine ni ikizamini cyo kumenya cyangwa kugereranya endotoxine ikomoka kuri bagiteri-mbi.

Inkwavu zikoreshwa mugushakisha no kugereranya endotoxine mubicuruzwa bya farumasi.Nk’uko USP ibitangaza, RPT ikubiyemo gukurikirana niba ubushyuhe bwiyongera cyangwa umuriro nyuma yo gutera imiti mu nkwavu.Kandi 21 CFR 610.13 (b) isaba urukwavu rwa pyrogene kubicuruzwa byibinyabuzima byihariye.

Mu myaka ya za 1960, Fredrick Bang na Jack Levin basanze amebocytes yo mu gikona cy'amafarashi izambara imbere ya endotoxine.UwitekaLimulus Amebocyte Lysate(cyangwa Tachypleus Amebocyte Lysate) yatejwe imbere kugirango isimbuze RPT nyinshi.Kuri USP, ikizamini cya LAL cyitwa testi ya bacteri endotoxine (BET).Kandi BET irashobora gukorwa hakoreshejwe tekinike 3: 1) tekinike ya gel-yambaye;2) tekinike ya turbidimetric;3) tekinike ya chromogenic.Ibisabwa kubizamini bya LAL birimo pH nziza, imbaraga za ionic, ubushyuhe, nigihe cyo gukora.

Ugereranije na RPT, BET irihuta kandi neza.Ariko, BET ntishobora gusimbuza RPT burundu.Kuberako LAL isuzuma rishobora kubangamirwa nibintu kandi ntishobora kumenya pyrogène itari endotoxine.